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4. Genetic Mutations and Gene Editing
About this Lecture
In the fourth mini-lecture, we address how genes can be edited. To do so, we first begin by defining what a gene is, by understanding its place in the genome, how it is made up of codons in DNA, where it can be found in the cell, and how it codes for proteins. We then move on by understanding how genes can be mutated by understanding deletion, insertion and substitution. We learn about the effects that just one mutation can have on a cell and its structure by comparing healthy cells with mutated cells, before understanding how modern technologies in gene editing can revert diseased cells back to healthy ones. CRISPR/Cas9 gene editing is one of the most powerful and innovative of these technologies, and in this lecture we continue by learning its step-by-step process.
In this lecture, Dr James Smith (University of East Anglia) introduces us to the use of stem cells in modern science. To do so, we begin by: (i) introducing the main features of a stem cells and their hierarchy; before (ii) looking at how Yamanaka factors can be manipulated to reprogram specialised cells back into stem cells; and then (iii) moving into the laboratory to demonstrate some of the ways in which this is done; following onto (iv) understanding the gene, and how it can be mutated and edited; and then (v) understanding important experimental techniques geneticists use such as PCR, gel electrophoresis and recombinant DNA; and finally (vi) discuss some of the ramifications of using stem cells and editing genes.
Dr James G. Smith is a scientific group leader working with pluripotent stem cells at Norwich Medical School, University of East Anglia, UK. He completed his PhD at the University of Birmingham with a focus on mesenchymal stem cells and the extracellular matrix. He then undertook post-doctoral training at the University of Nottingham developing automated manufacture technologies for human pluripotent stem cells. He was later appointed as a senior research fellow, using CRISPR gene editing and hiPSCs as tools to investigate cardiovascular disease, before setting up my own group at UEA in 2019
Cite this Lecture
Smith, J. (2022, August 30). Stem Cells and Gene Editing - Genetic Mutations and Gene Editing [Video]. MASSOLIT. https://massolit.io/courses/stem-cells-and-gene-editing/genetic-mutations-and-gene-editing
Smith, J. "Stem Cells and Gene Editing – Genetic Mutations and Gene Editing." MASSOLIT, uploaded by MASSOLIT, 30 Aug 2022, https://massolit.io/courses/stem-cells-and-gene-editing/genetic-mutations-and-gene-editing