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3. Culturing Stem Cells
About this Lecture
In the third mini-lecture, we move into the laboratory to learn how scientists culture stem cells into specialised cells. We begin by learning how they are kept, and how we can monitor them. To continue, we learn how stem cells are ‘fed’ using a special fluid known as culture media, containing factors that reprogram cells into specialised cells, whilst also providing them with the right amount of nutrition. In the final part, we are shown a sample of heart cells (which were originally induced pluripotent stem cells) ‘beating’ on camera.
In this lecture, Dr James Smith (University of East Anglia) introduces us to the use of stem cells in modern science. To do so, we begin by: (i) introducing the main features of a stem cells and their hierarchy; before (ii) looking at how Yamanaka factors can be manipulated to reprogram specialised cells back into stem cells; and then (iii) moving into the laboratory to demonstrate some of the ways in which this is done; following onto (iv) understanding the gene, and how it can be mutated and edited; and then (v) understanding important experimental techniques geneticists use such as PCR, gel electrophoresis and recombinant DNA; and finally (vi) discuss some of the ramifications of using stem cells and editing genes.
Dr James G. Smith is a scientific group leader working with pluripotent stem cells at Norwich Medical School, University of East Anglia, UK. He completed his PhD at the University of Birmingham with a focus on mesenchymal stem cells and the extracellular matrix. He then undertook post-doctoral training at the University of Nottingham developing automated manufacture technologies for human pluripotent stem cells. He was later appointed as a senior research fellow, using CRISPR gene editing and hiPSCs as tools to investigate cardiovascular disease, before setting up my own group at UEA in 2019
Cite this Lecture
Smith, J. (2022, August 30). Stem Cells and Gene Editing - Culturing Stem Cells [Video]. MASSOLIT. https://massolit.io/courses/stem-cells-and-gene-editing/culturing-stem-cells
Smith, J. "Stem Cells and Gene Editing – Culturing Stem Cells." MASSOLIT, uploaded by MASSOLIT, 30 Aug 2022, https://massolit.io/courses/stem-cells-and-gene-editing/culturing-stem-cells